RBP Type	Non-canonical_RBPs
Diseases	N.A.
Drug	N.A.
Main interacting RNAs	N.A.
Moonlighting functions	Metabolic enzyme
Localizations	N.A.
BulkPerturb-seq

Ensembl ID	ENSG00000065911	Gene ID	10797	Accession	7434
Symbol	MTHFD2	Alias	NMDMC	Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Status	Confidence	Length	31394 bases	Strand	Plus strand
Position	2 : 74186172 - 74217565	RNA binding domain	N.A.
Summary	This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

Protein	Domain	Pfam ID	E-value	Domain number

Pubmed ID	Full Name	Cell	Author	Time	Doi

Pubmed ID	Title	Author	Time	Journal
30275950	Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.	Costas Koufaris	2018-01-01	Cancer & metabolism
32439610	Reprogramming of serine, glycine and one-carbon metabolism in cancer.	M Albert Li	2020-10-01	Biochimica et biophysica acta. Molecular basis of disease
32411609	More Than a Metabolic Enzyme: MTHFD2 as a Novel Target for Anticancer Therapy?	Zhiyuan Zhu	2020-01-01	Frontiers in oncology
35054987	The Epithelial-Mesenchymal Transition at the Crossroads between Metabolism and Tumor Progression.	Monica Fedele	2022-01-12	International journal of molecular sciences

Name	Transcript ID	bp	Protein	Translation ID
MTHFD2-213	ENST00000677997	3340	324aa	ENSP00000503074
MTHFD2-209	ENST00000677170	3362	248aa	ENSP00000503486
MTHFD2-218	ENST00000678684	2710	248aa	ENSP00000504687
MTHFD2-203	ENST00000409804	1757	222aa	ENSP00000386536
MTHFD2-205	ENST00000470592	1396	37aa	ENSP00000419067
MTHFD2-208	ENST00000489041	2463	38aa	ENSP00000419550
MTHFD2-220	ENST00000678955	6506	No protein	-
MTHFD2-206	ENST00000477455	696	No protein	-
MTHFD2-215	ENST00000678340	2057	212aa	ENSP00000503933
MTHFD2-221	ENST00000679055	2276	248aa	ENSP00000503701
MTHFD2-211	ENST00000677377	4033	36aa	ENSP00000502903
MTHFD2-201	ENST00000394053	4403	350aa	ENSP00000377617
MTHFD2-202	ENST00000409601	1865	267aa	ENSP00000386542
MTHFD2-214	ENST00000678228	2320	38aa	ENSP00000503299
MTHFD2-207	ENST00000488086	483	No protein	-
MTHFD2-204	ENST00000462026	895	203aa	ENSP00000503386
MTHFD2-216	ENST00000678623	1834	248aa	ENSP00000504392
MTHFD2-219	ENST00000678731	2309	248aa	ENSP00000503927
MTHFD2-210	ENST00000677299	2438	No protein	-
MTHFD2-212	ENST00000677558	1243	36aa	ENSP00000503573
MTHFD2-217	ENST00000678633	1239	36aa	ENSP00000504822

Pathway ID	Pathway Name	Source

ensgID	Trait	pValue	Pubmed ID

ensgID	SNP	Chromosome	Position	Trait	PubmedID	Or or BEAT	EFO ID

Ensembl ID	Source				Target
	Species	Protein ID	Perc_pos	Perc_id	Species	Protein ID	Perc_pos	Perc_id

Ensembl ID	Source				Target
	Species	Protein ID	Perc_pos	Perc_id	Species	Protein ID	Perc_pos	Perc_id

Go ID	Go term	No. evidence	Entries	Species	Category
GO:0000287	enables magnesium ion binding	2	IDA,IMP	Homo_sapiens(9606)	Function
GO:0004477	enables methenyltetrahydrofolate cyclohydrolase activity	4	IBA,IDA,ISS,TAS	Homo_sapiens(9606)	Function
GO:0004487	enables methylenetetrahydrofolate dehydrogenase (NAD+) activity	4	IBA,IDA,IMP,TAS	Homo_sapiens(9606)	Function
GO:0004488	enables methylenetetrahydrofolate dehydrogenase (NADP+) activity	3	IBA,IDA,IMP	Homo_sapiens(9606)	Function
GO:0005515	enables protein binding	1	IPI	Homo_sapiens(9606)	Function
GO:0005615	located_in extracellular space	1	HDA	Homo_sapiens(9606)	Component
GO:0005739	is_active_in mitochondrion	3	IBA,IDA,ISS	Homo_sapiens(9606)	Component
GO:0005759	located_in mitochondrial matrix	1	TAS	Homo_sapiens(9606)	Component
GO:0035999	involved_in tetrahydrofolate interconversion	1	IBA	Homo_sapiens(9606)	Process
GO:0042301	enables phosphate ion binding	2	IDA,IMP	Homo_sapiens(9606)	Function
GO:0046653	involved_in tetrahydrofolate metabolic process	1	IDA	Homo_sapiens(9606)	Process
GO:0046655	involved_in folic acid metabolic process	1	TAS	Homo_sapiens(9606)	Process